Part:BBa_K2220027:Design

Express beta-tubulin-mGFP fusion protein

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 248
  Illegal BamHI site found at 1031
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2128
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2110
  Illegal BsaI.rc site found at 592
  Illegal BsaI.rc site found at 1994
  Illegal SapI site found at 1128
  

Design Notes

We have done the codon optimization.

Source

Beta-tubulin: http://www.ncbi.nlm.nih.gov/nucleotide/645912969